Gene Amplification (PCR)

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Gene Amplification (PCR): Overview

This topic covers concepts, such as, Polymerase Chain Reaction,Mechanism of PCR etc.

Important Questions on Gene Amplification (PCR)

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The source organisms that possess Taq polymerase are

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In which of the following technique the bacterium Thermus aquatics is used:

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Colonies of recombinant bacteria appear white in contrast to non-recombinant bacteria because of 

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_____ are the short nucleiotide sequences that are complementary to the ends of the DNA streches that needs to be amplified. 

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The technique associated with DNA amplification is _____. (Polymerase chain reaction / Western blotting)

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The correct order of steps involved in PCR technique is:

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The DNA extracted from elution can be amplified by PCR.

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What is the genus name of bacterium that yields a Taq DNA polymerase?

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Name the technique that is used to make lacs of copies of DNA from a single copy in very limited time. (Write the full form of it.)

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Which among the following does not help in early diagnosis?

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Describe gene amplification (PCR). 

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PCR technique was developed by ____.

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Explain Annealing.

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Explain the denaturation of DNA. 

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Describe the steps that constitute one cycle of reactions in PCR. 

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Write comments on Polymerase Chain Reaction.

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In 1989, Mullis discovered

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Select the option in which the steps of PCR are arranged in the right order:

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The enzyme Taq polymerase used in PCR technique is obtained from ________

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During PCR technique, the pairing of primers to ssDNA segment is called